Composite

Part:BBa_K3734022:Design

Designed by: Xingjun Zhao   Group: iGEM21_CSU_CHINA   (2021-10-01)


TRE-mcherry-miR21


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1079
    Illegal NotI site found at 1085
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1055
    Illegal XhoI site found at 1
    Illegal XhoI site found at 1739
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2819


Design Notes

Because miR21 need to be processed in front of the mature, it need to be insert into a special location.


Source

TRE is from E.coli. The miR21 is from human genomes.

References